柔嫩艾美耳球虫感染对京海黄鸡不同组织中Zyxin、CD4和TNFRSF1A 基因mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2014.08.024

Abstract

Abstract:

In order to analyze the effect of Emeria tenella infection on the expression of Zyxin，CD4 and TNFRSF1A in Jinghai Yellow chickens，the expression of the three genes in 10 tissues were detected by fluorescent quantitative PCR.The results showed that the expression of the 3 genes were significantly different between the infected and control groups in most tissues.Zyxin expression in glandular stomach was the highest among detected tissues in infected group，except for in lung，liver，kidney and cardiac muscle，the expression of infected group was significantly higher than that of control group(P<0.01)；CD4 expression of infected group in some immune organs，such as spleen，lung and thymus，was significantly higher than that of control group(P<0.01)；TNFRSF1A expressions in small intestine and caecum were at high level，and was significantly higher in infected group than that in control group(P<0.01) in small intestine，caecum，spleen，glandular stomach and thymus gland；Three gene expressions in bursa of Fabricius were at low level，and none significant difference was found between the infected and control groups except for Zyxin，which suggested that bursa of Fabricius probably did not play an important role in the infection of Emeria tenella.In caecum，the parasitic site of Emeria tenella，the expression of Zyxin and TNFRSF1A of infected group had significant difference with the control group(P＜0.01)，which indicate that the two genes had a close relationship with the infection of Emeria tenella.

CLC Number:

S831.2

SUN Ming-ming，LIN Yu-xin，DAI Guo-jun，ZHANG Jing-jing，ZHANG Gen-xi，XIE Kai-zhou，WANG Jin-yu，GU Yu-ping，WANG Yong-juan. The Effect of Emeria tenella Infection on the Expression of Zyxin，CD4，TNFRSF1A in Different Tissues of Jinghai Yellow Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1369-1374.

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The Effect of Emeria tenella Infection on the Expression of Zyxin，CD4，TNFRSF1A in Different Tissues of Jinghai Yellow Chicken

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